The exponential increase in the number of researchers exploring the intricacies of stem cell biology make this an exciting time. The ability of stem cells to differentiate into multiple lineages allows them to be used in ways that have the potential to dramatically improve the human condition. The recent explosion in stem cell research can be attributed to the isolation of human embryonic stem cells. However, the increased interest in these cells has also highlighted the limitations of current technology. Therefore, as the field progresses toward its ultimate applications in drug discovery and regenerative medicine, several hurdles need to be overcome. The future pace of progress in stem cell research will therefore largely depend on the development of new technologies that can either overcome or minimize current constraints.
The idea for this book was conceived as a result of our unending obsession in working with stem cells. Most techniques and protocols used in stem cell research continue to be more of an art than science, and this has been a source of constant frustration for stem cell researchers across the world. In this book, we present some of the elegant studies that have translated technical skill into a scientific process. We have attempted to bring to the forefront some of the emerging technology platforms and their applications in stem cell research.
Above all, this book is an ode to stem cell researchers who are involved in painstakingly assembling the small pieces to create a larger picture. We are sincerely thankful to all the contributors for sharing their pioneering work and scientific perspectives in this book. Our hope is that this book will ignite a passion in people who are new to the field while also engaging researchers currently working on different aspects of stem cell biology. Our belief is that the technologies described in this book will have far - reaching effects in the field of stem cell research, and will help accelerate the path to the clinic. The purpose of this book is to provide a realistic picture of the potential and challenges of stem cell research as it continues its trailblazing journey toward the quest for a better human life.
- The Authors -
Book contents
Part 1
- Chapter 1: Derivation methods for human embryonic stem cells: Past, present & future
- Chapter 2: Isolation of human ESCs from various stages of the human embryo
- Chapter 3: Derivation of stem cells from epiblasts
- Chapter 4: Derivation of Embryonic Stem Cells from Parthenogenetic Eggs
- Chapter 5: Reprogramming Developmental Potential
Part 2
- Chapter 6: Adult stem cells and their role in endogenous tissue repair
- Chapter 7: Greater differentiation potential of adult stem cells
- Chapter 8: Cancer stem cells
- Chapter 9: Large scale production of adult stem cells for clinical use
Part 3
- Chapter 10: Genetic and epigenetic features of stem cells
- Chapter 11: Directed differentiation of embryonic stem cells
- Chapter 12: Identification of signaling pathways involved during differentiation
Part 4
- Chapter 13 Media and extracellular matrix requirements for large scale ESC growth
- Chapter 14: Automated method for culturing ES cells
- Chapter 15: Quantitative 2D Imaging of Human Embryonic Stem Cells
- Chapter 16: Nanobiotechonology for stem cell culture and Maintenance
- Chapter 17: Engineering Microenvironments to Control Stem Cell Functions
Part 5
- Chapter 18: Improved lentiviral gene delivery tools for stem cells
- Chapter 19: Sleeping Beauty-mediated Transposition in Stem Cells
- Chapter 20: PhiC31 Integrase for Modification of Stem Cells
- Chapter 21: Cell Engineering using Integrase and Recombinase systems
Part 6
- Chapter 22: hESC derived cardiomyocytes for cell therapy and drug discovery
- Chapter 23: hESC in Drug discovery
- Chapter 24: Characterization and Culturing of Adipose-Derived Precursor Cells
- Chapter 25: Bringing Mesenchymal stem cells to clinic
Index
About the Authors
- Uma Lakshmipathy is a Principal Scientist at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds two patents and has authored over thirty publications.
- Jonathan D. Chesnut is an R & D Director at Life Technologies, where he heads the stem cell engineering group. He holds five patents and has authored over twenty publications.
- Bhaskar Thyagarajan is a Program Manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored over twenty publications.
Book Review
Although several attempts are ongoing, I believe this textbook authored by my colleagues is the first effort to see the light of print. It is truly comprehensive in covering all major aspects of the field. Most of the chapters have been written by luminaries in the field and the editing has ensured a consistent and direct style. It seems to this reader that every effort has been made to include the latest results and identify the latest references.
I was impressed by the book and I hope others will find it as informative and useful as I do.
I congratulate the editors on a job well done.
by Mahendra Rao
Faculty, Buck Institute of Aging, Novato, California
VP, Stem Cell & Regenerative Medicine, Invitrogen Corporation, Carlsbad, CA.
Product Details
- Hardcover: 521 pages
- Publisher: Wiley; 1 edition (April 6, 2009)
- Language: English
- ISBN-10: 0470146931
- ISBN-13: 978-0470146934
- Product Dimensions: 6.5 x 1.2 x 9.4 inches
List Price: $132.00