In the Fifth Edition, authors Dave Nelson and Mike Cox combine the best of the laboratory and best of the classroom, introducing exciting new developments while communicating basic principles through a variety of new learning tools—from new in-text worked examples and data analysis problems to the breakthrough eBook, which seamlessly integrates the complete text and its media components.
New to this edition
MAJOR RECENT ADVANCES IN BIOCHEMISTRY
Thoroughly revised throughout, the new edition incorporates new research on the
expanded role of RNA in biology, hormonal regulation of metabolism and its relation to obesity and diabetes, new methods in bioinformatics, and the application of biochemistry to medicine, forensics, and agriculture. New discussions include:
- Introduction to proteomes and proteomics (Chapter 1)
- Amyloid diseases in the context of protein folding (Chapter 4)
- Pharmaceuticals developed from an understanding of enzyme mechanism, using penicillin and HIV protease inhibitors as examples (Chapter 6)
- Sugar analogs as drugs that target viral neuraminidase (Chapter 7)
- Green fluorescent protein (Chapter 9)
- Lipidomics (Chapter 10)
- Volatile lipids used as signals by plants, and pigments of bird feathers derived from colored lipids in plant foods (Chapter 10)
- Lipid rafts and caveolae (Chapter 11)
- The emerging role of ribulose 5-phosphate as central regulator of glycolysis and gluconeogenesis (Chapter 15)
- Moonlighting Enzymes: Proteins with More Than One Job (Box 16-1)
- The role of transcription factors (PPARs) in regulation of lipid catabolism (Chapter 17)
- Fatty acid synthase, including new structural information on FAS I (Chapter 21)
- Epigenetics, Nucleosome Structure, and Histone Variants, on the role of histone modification and nucleosome deposition in the transmission of epigenetic information in heredity (Box 24-2)
- Initiation of replication and the dynamics at the replication fork, introducing AAA+ ATPases and their functions in replication and other aspects of DNA metabolism (Chapter 25)
- The roles of RNA in cells (Chapter 26)
- The roles of RNA in protein biosynthesis (Chapter 27)
- Riboswitches (Chapter 28)
- Of Fins, Wings, Beaks, and Things, on the connections between evolution and development (Box 28-1)
NEW BIOCHEMICAL METHODS
Some of the new or updated methods described in the new edition include:
- Circular dichroism (Chapter 4)
- Measurement of glycated hemoglobin as an indicator of average blood glucose concentration over several days (Chapter 7)
- The use of MALDI-MS in the determination of oligosaccharide structure (Chapter 7)
- Forensic DNA analysis, including modern STR analysis (Chapter 9)
- Microarrays (Chapter 9)
- Use of tags for protein analysis and purification (Chapter 9)
- Positron emission tomography (PET) combined with CT scan to pinpoint cancer (Chapter 14)
- Chromatin immunoprecipitation and ChIP-chip experiments (Chapter 24)
- The development of bacterial strains with altered genetic codes to insert novel amino acids site-specifically in proteins (Chapter 27)
UNDERSTANDING METABOLISM THROUGH OBESITY AND DIABETES
This edition’s focus on the interplay of metabolism, obesity, and diabetes provides an integrating theme for the chapters on metabolism and its control. Highlights include:
- Untreated Diabetes Produces Life-Threatening Acidosis (Chapter 2)
- Blood Glucose Measurements in the Diagnosis and Treatment of Diabetes (Box 7–1)
- Defective Glucose and Water Transport in Two Forms of Diabetes (Box 11–2)
- Glucose Uptake Is Deficient in Type 1 Diabetes Mellitus (Chapter 14)
- Ketone Bodies Are Overproduced in Diabetes and during Starvation (Chapter 17)
- Mitochondrial diseases and mitochondrial role in diabetes (Chapter 19)
- Diabetes Can Result from Defects in Mitochondria of Pancreatic b Cells (Chapter 19)
- Adipose Tissue Generates Glycerol 3-phosphate by Glyceroneogenesis (Chapter 21)
- Diabetes Mellitus Arises from Defects in Insulin Production or Action (Chapter 23)
- Obesity and the Regulation of Body Mass discusses the role of adiponectin and insulin sensitivity and type 2 diabetes (Section 23.4)
- Obesity, the Metabolic Syndrome, and Type 2 Diabetes includes a discussion of managing type 2 diabetes with exercise, diet, and medication (Section 23.5)
MORE MEDICALLY RELEVANT EXAMPLES
- Throughout the new edition, this icon denotes material of special medical interest. New examples include:
- The role of polyunsaturated fatty acids and trans fatty acids in cardiovascular disease (Chapter 10)
- G protein–coupled receptors (GPCRs) and the range of diseases for which drugs target GPCRs (Chapter 12)
- G proteins, the regulation of GTPase activity, and the medical consequences of defective G protein function (Chapter 12)
- The use of protein kinase inhibitors in cancer therapy (Chapter 12)
- The high rate of glycolysis in cancerous tissue aids in the diagnosis of cancer and suggests targets for chemotherapy (Chapter 14)
- Mutations that lead to rare forms of diabetes (MODY) (Chapter 15)
- Mutations in citric acid cycle that lead to cancer (Chapter 16)
- Pernicious anemia and associated problems in strict vegetarians (Chapter 18)
- Updated information on cyclooxygenase inhibitors (pain relievers Vioxx, Celebrex, Bextra) (Chapter 21)
- HMG-CoA reductase and a new box on statins (Chapter 21)
- The use of topoisomerase inhibitors in the treatment of bacterial infections and cancer, including material on ciprofloxacin (the antibiotic effective for anthrax) (Chapter 24)
NEW PROBLEM SOLVING TOOLS
- New In-text Worked Examples help students improve their quantitative problem-solving skills by taking them through difficult equations
- Over 100 New End-of-chapter Problems give students the opportunity to practice what they have learned
- New Data Analysis Problems (one at the end of each chapter, contributed by Brian White of the University of Massachusetts-Boston) encourage students to synthesize what they have learned and apply their knowledge to the interpretation of data from the literature
NEW TREATMENT OF FUNDAMENTAL CONCEPTS
- A new section, 13.2: Chemical Logic and Common Biochemical Reactions, discusses the common biochemical reaction types that underlie all metabolic reactions
- Chemical Logic is reinforced in the discussions of central metabolic pathways
- Mechanism figures feature step-by-step callouts to help students understand the reaction process and use a consistent set of conventions introduced in detail with the first enzyme mechanism encountered
- Key Conventions, critical for understanding each biochemical topic and the biochemical literature, are now highlighted. These include clear statements of many assumptions and conventions that studentsare often expected to assimilate without being told (e.g., peptide sequences are presented amino- to carboxyl -terminal, left to right; nucleotide sequences are presented 5’ to 3’, left to right, etc.)
Product Details
- Hardcover: 1100 pages
- Publisher: W. H. Freeman; 5th edition (February 1, 2008)
- Language: English
- ISBN-10: 071677108X
- ISBN-13: 978-0716771081
- Product Dimensions: 10.9 x 8.8 x 1.7 inches
List Price: $161.99