Since the publication of the second edition of Comprehensive Cytopathology in 1997 new trends in the practice of gynecologic and non gynecologic cytology have emerged. Liquid based preparations and HPV testing have been introduced in gynecologic cytology, immunocytochemistry, clinical cytogenetics and molecular techniques have seen expanded applications to non gynecologic cytology, and fine needle aspiration under sonographic, computer tomographic or fluoroscopic guidance for cytologic diagnosis has become an indispensable component of the workup of body lesions, with more FNA's being performed by radiologists, and endoscopists in close cooperation with cytologists.
The table of contents reflects additions and changes in topics to provide detailed discussions of the significant advances in the field. Examples include New concepts on molecular basis of neoplasia (chapter 1), The role of genetics in cancer development (chapter 2), Evaluation of the sample in smears and liquid based preparations (chapter 5), FNA of Mediastinum (chapter 26), FNA of Pediatric Tumors (chapter 29), Virtual Microscopy (chapter 33), Automation of Cervical Cytology (chapter 34), Molecular Techniques (chapter 36). Among the unique features of this edition is the large number of more than 1700 color illustrations depicting the main diagnostic entities with applications of adjunct techniques to cytopathology. New contributors from institutions around the world (chapters 1, 2, 3, 6, 9, 10, 18, 21, 24, 25, 26, 28, 29, 33, 34, 35 & 36) have joined the distinguished roster of authors not only to write new chapters but also to rewrite them.
I would like to thank Dr David Wilbur for editing the chapters in the FGT section of CC3E and for his valuable input. Thanks to all contributors for their efforts and outstanding contributions. Special thanks to Nani Clansey, Development Editor, for her enormous help in the development of this work and William R Schmitt, Editor, for his support and also to Rory MacDonald, Project Manager, Stewart Larking, Designer and to Gillian Richards, Illustration Manager in Elsevier Ltd.
In this third edition of Comprehensive Cytopathology we have attempted, through an international group of experts to present a state of the art work. Again we placed in a single comprehensive book discussions on general cytology, diagnostic exfoliative cytology of all body sites, entities and diagnostic challenges in fine needle aspiration of various organs, effects of therapy on cytologic specimens and special methodologies and technologies in cytology. We hope that our attempt to incorporate advances in the field while keeping emphasis on cytomorphology will be valuable to all professionals interested in cytopathology.
-- Marluce Bibbo, MD
Key Features
- Provides an accessible guide to diagnostic investigation and screening.
- Includes a summary of major diagnostic criteria and discusses the pitfalls and limitations of cytology.
- Utilizes a consistent chapter structure to make finding the answers you need quick and easy.
Website Features
- Consult the book from any computer at home, in your office, or at any practice location.
- Instantly locate the answers to your clinical questions via a simple search query.
- Quickly find out more about any bibliographical citation by linking to its MEDLINE abstract.
- Path Consult DDX Fundamentals Maximize clinical efficiency and productivity with Path Consult, an easy-to-use online resource that will help you quickly and easily confirm diagnoses.
Contents
Part I - General Cytology
Chapter 1 - The Cell: Basic Structure And Function
- Basic Structure And Function Of Mammalian Cells
- The Molecular Basis Of Neoplasia
Chapter 2 - Basic Cytogenetics And The Role Of Genetics In Cancer Development
- Introduction
- Historical Background
- Basic Knowledge Of Cytogenetics
- Methodology
- Acquired Chromosomal Aberrations In Cancer
- Clinical Applications Of Conventional Cytogenetics And Fish In Cytology
- Concluding Remarks
Chapter 3 - Cytologic Screening Programs
- Principles Of Screening
- Cervical Cancer And Screening
- Early Detection Of Cancer In Other Sites
- New Developments In Cytological Screening
Chapter 4 - Diagnostic Quality Assurance In Cytopathology
- Introduction
- Quality Assurance Measures
- Internal Quality Assurance Mechanisms
- Computer-Assisted Quality Assurance Mechanisms
- External Quality Assurance Mechanisms
- Continuing Education Practices
Chapter 5 - Evaluation Of The Sample In Smears And Liquid-Based Preparations
- Introduction
- Cervicovaginal Cytology
- Nongynecologic Cytology
Part II - Diagnostic Cytology
Chapter 6 - The Bethesda System for Reporting Cervical Cytology
- The Bethesda System: Historical Perspective
- Report Format
- Specimen Adequacy
- General Categorization
- Interpretation/Result
- Educational Notes/Suggestions
- Ancillary Testing
- Automated Review
- Interobserver Reproducibility in Cervical Cytology
- The Bethesda System and Reporting Anal-Rectal Cytology
Chapter 7 - Microbiology, Inflammation and Viral Infections
Chapter 8 - Benign Proliferative Reactions, Intraepithelial Neoplasia, and Invasive Cancer of the Uterine Cervix
Chapter 9 - Glandular Lesions of the Uterine Cervix
Chapter 10 - Endometrial Lesions, Unusual Tumors and Extrauterine Cancer
Chapter 11 - Vulva, Vagina, and Anus
Chapter 12 - Peritoneal Washings and Ovary
Chapter 13 - Respiratory Tract
Chapter 14 - Alimentary Tract (Esophagus, Stomach, Small Intestine, Colon, Rectum, Anus, Biliary Tract)
Chapter 15 - Urinary Tract
Chapter 16 - Central Nervous System
Chapter 17 - Eye
Chapter 18 - Cytology of Soft Tissue, Bone, and Skin
Chapter 19 - Pleural, Peritoneal, and Pericardial Effusions
Chapter 20 - Fine-Needle Aspiration Biopsy Techniques
Chapter 21 - Imaging Techniques
Chapter 22 - Salivary Glands and Rare Head and Neck Lesions
Chapter 23 - Thyroid
Chapter 24 - Lymph Nodes: Cytomorphology and Flow Cytometry
Chapter 25 - Breast
Chapter 26 - Mediastinum
Chapter 27 - Kidneys, Adrenals, and Retroperitoneum
Chapter 28 - Liver and Pancreas
Chapter 29 - Pediatric Tumors
Chapter 30 - Effects of Therapy on Cytologic Specimens
Part III - SPECIAL TECHNIQUES IN CYTOLOGY
Chapter 31 - Cytopreparatory Techniques
- Introduction
- Fixation
- General Comments on Fixation
- Fixation Methods
- Air Drying for Selected Cell Samples
- Other Considerations
- Papanicolaou Staining Methods
- Staining Procedures Based on the Papanicolaou Method
- Counterstains: Orange G and EA
- Environmentally Friendly Staining Procedure
- On-Site Quick Staining Procedure
- Helpful Hints When Staining with the Papanicolaou Method
- Troubleshooting the Papanicolaou Staining Methods
- Cross-Contamination Method to Avoid Floaters
- Cover Slipping
- Labeling
- Automated Cover Slipping Procedure
- Liquid Mounting Media
- Destaining and Restaining Slides
- Collecting and Processing Nongynecologic Cell Samples
- Respiratory Tract Specimens and Other Mucoid Samples
- Staining Body Fluids
- Infection Control
- Other Factors Related to Cytopreparation
- Concluding Remarks
Chapter 32 - Light Optical Microscopy
- Introduction
- Image Formation
- Koehler Illumination
- Concluding Remarks
Chapter 33 - Virtual Microscopy
- Virtual Microscopy
- Virtual Slide Acquisition
- Equipment for Slide Acquisition
- Acquisition Speed
- Virtual Slide Quality
- Focus Planes
- Applications
- Teaching: Online Education
- Diagnostics
- Teleconsultation
- Quality Assurance
- Data Storage
- Research
Chapter 34 - Automation in Cervical Cytology
- Introduction
- Historical Attempts at Automation
- The Rationale for Automation
- Cytology Automation: Accuracy and Productivity
- Currently Available Automation Platforms
- Liquid-based Preparation
- Automated Screening Devices
- Laboratory Process Issues Associated with the Use of Automated Devices
- Reporting Issues
- Issues with Specimens that Cannot Be Successfully Processed
- Stain Use in Automated Systems
- Training Required for Initiation of Automated Methods
- Laboratory Workflow Issues
- Cost-effectiveness of Liquid-based Preparation and Automated Screening Devices
Chapter 35 - Immunocytochemistry
- Introduction
- Immunocytology Techniques: The Specimen
- Interpretation and Limitations of ICC
- Effusion Cytology
- Mesothelial Markers
- Calretinin
- HBME-1
- Cytokeratin-5/6
- Wilms' Tumor Gene Product
- D2-40
- GLUT-1
- Non Mesothelial (Adenocarcinoma) Markers
- MOC-31
- BG-8
- Ber-EP4
- Monoclonal CEA
- TAG-72.3
- CD15 (Leu-M1)
- Site-specific Markers
- Thyroid Transcription Factor-1
- Estrogen Receptor
- CDX2
- Breast Cytology: E-cadherin and p120 Catenin
- Immunocytochemistry for Targeted Therapies
- CD117
- HER2/neu
- Epidermal Growth Factor Receptor
- Gynecologic Cytology
- p16ink4a
- ProEx C
- In Situ Hybridization Assays for Detection of High-Risk HPV
- Ovarian Cytology
- Wilms' Tumor Gene Product
- Sox-9
- Hepatocyte Nuclear Factor-1β
- Carcinoma of Unknown Primary
- Nonepithelial Malignancies Presenting as Tumors of Unknown Origin
- Malignant Melanoma
- Ki67
- c-met
Chapter 36 - Molecular Techniques
- Introduction
- Molecular Techniques in Cytopathology
- Fluorescence In Situ Hybridization
- Polymerase Chain Reaction
- Microsatellite Analysis/Loss of Heterozygosity
- Laser Microdissection
- Promoter Methylation Analysis
- Hybrid Capture System
- Applications in Cytology
- Improved Diagnosis and Classification of Cancer
- Other Applications
- Molecular Analysis of Therapeutic Targets
- HER2, TOP2A, and ESR1 in Breast Cancer
- EGFR Analysis in Non Small Cell Lung Cancer
- Human Papillomaviruses
- Classification of Human Papillomaviruses
- High-Risk and Low-Risk HPV Types
- Traditional DNA Techniques Used for HPV Detection
- Novel HPV Assays of the Third Millennium
- Detection of HPV DNA by Molecular Techniques
- Comparing PCR with the HC2 Assay
- FISH in Gynecologic Cytology
- Clinical Value of and Controversies in HPV Testing
About the Authors
- Marluce Bibbo, MD ScD FIAC FASCP, Professor of Pathology and Cell Biology, Jefferson Medical College, Director, Cytopathologym, Thomas Jefferson University Hospital, Philadelphia, PA, USA.
- David Wilbur, MD, Associate Professor of Pathology, Harvard Medical School, Director, Cytopathology, Massachusetts General Hospital, Boston, MA, USA.
Product Details
- Hardcover: 1136 pages
- Publisher: Saunders; 3 edition (2009)
- Language: English
- ISBN-10: 1416042083
- ISBN-13: 978-1416042082
- Product Dimensions: 11.1 x 9.1 x 2 inches
List Price: $410.00